Amino acids play central roles both as building blocks of proteins and as intermediates in metabolism. Optical biosensors are technically designed for the one particular target site and capable of recognizing a desire ligand molecule. For instance, GlyFs sensor engineered by Jackson lab, is a neurotransmitter and a metabolite bio-marker for Type-2 Diabetes (T2D). Diabetes is a serious complex disease and the complex interaction of genetic and environmental factors. The system can’t maintain the healthy levels of glucose in the blood. Type-2 diabetes accounts for 85 % of all diabetes and it’s gradually increasing with the undiagnosed (T2D) which damaging their bodies. Optical biosensors offer great advantages because they detect the label-free biosensors of many biological and chemical substances. Nanoscale structures which interacts strongly with local refractive index causes the shift in resonance frequency, which can be easily detected. In this work we are focusing on developing highly sensitive nano-scale structures for label free, non-invasive, point of care diagnostic devices with the aid of bio-markers, we can have selective sensor binding to the surface these leads to higher sensitivity and selectivity, which is highly desirable in sensing platforms. Initially, metasurfaces is functionalized as glycine sensor proteins to capture the analytes with microfluidics to perform dynamic sensing. As the ligand binds to the glycine binding protein occurs the conformational changes and this will be used to study the various concentrations of glycine to detect the bio-marker for diabetes.