Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a key technology that provides
critical information on both protein conformation and dynamics. In recent years, HDX-MS has
become an indispensable tool in the biopharmaceutical industry to answer important questions
concerning biotherapeutic structure, stability, and interactions. In this study, we used HDX-MS to
compare the higher order structure similarity between innovator and biosimilar versions of a mAb.
Our results show that the structures of the innovator and biosimilar mAbs are comparable.
However, minute differences were observed in the Fc-CH2 domain and a few overlapping
peptides in this region attracted our attention. Details will be discussed in the poster.